Dr Richard D Antrobus
|Technology Exchange:||Cellular immunology, Transcript profiling and Vaccine production and evaluation|
|Keywords:||Immunology and Influenza vaccine trials|
Richard came from a background in Clinical Immunology to join the Jenner Institute in 2010. As a clinical research fellow he has been working with Professor Sarah Gilbert as the lead clinician on influenza vaccine trials using MVA-NP+M1. He has subsequently begun studying for a DPhil, focussing on differential gene expression during influenza challenge.
|Prof Sarah C Gilbert||Jenner Institute||Oxford University||UK|
|Prof Adrian VS Hill||Jenner Institute||Oxford University||UK|
|Dr Ellie (Eleanor) Barnes||Experimental Medicine Division||Oxford University||UK|
|Prof Paul Klenerman||Experimental Medicine Division||Oxford University||UK|
Br J Biomed Sci, 68 (2), pp. 98-99.2011. Optimising pre-analytical performance of interferon-gamma release assays for TB exposure.
Leukocyte immunoglobulin-like receptor-1 (LIR-1) is an inhibitory receptor that negatively regulates T cell effector functions after interaction with host class I major histocompatibility complex molecules and, additionally, binds to UL18, a human cytomegalovirus (HCMV)-encoded class I homologue. Here, we demonstrate that virus-specific cytotoxic T lymphocytes (CTLs) differentially express LIR-1, with high frequencies of expression on HCMV-specific CD8+ T cells and intermediate and low frequencies of expression on influenza virus-specific and Epstein-Barr virus (EBV)-specific CTLs, respectively. Expression of LIR-1 was dependent on CTL-antigen specificity and was associated with a differentiated effector memory phenotype, as demonstrated by decreased expression of CD28 and increased expression of CD57. During primary HCMV and EBV infections, expression of LIR-1 on virus-specific CTLs was low and increased slowly. These results indicate that expression of LIR-1 increases during differentiation of virus-specific CD8+ effector T cells. Furthermore, they suggest that a potential immunoregulatory function of UL18 may be to preferentially target highly differentiated HCMV-specific effector memory T cells during persistent infection. Hide abstract