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We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity to the reference genome (MHOM/GT2001/U1103). Through RNAseq-based annotation of coding sequences and untranslated regions, we provide primer sequences for construct and sgRNA template generation for CRISPR-assisted gene deletion and endogenous tagging.

Original publication

DOI

10.12688/wellcomeopenres.18575.1

Type

Journal article

Journal

Wellcome open research

Publication Date

01/2022

Volume

7

Addresses

Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.