Accuracy of Enzyme-Linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis
Chantratita N., Wuthiekanun V., Thanwisai A., Limmathurotsakul D., Cheng AC., Chierakul W., Day NPJ., Peacock SJ.
<jats:title>ABSTRACT</jats:title> <jats:p>Five enzyme-linked immunosorbent assays developed to detect antibodies to different <jats:italic>Burkholderia pseudomallei</jats:italic> antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude <jats:italic>B. pseudomallei</jats:italic> antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).</jats:p>