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OBJECTIVES: There is increasing interest in the use of metagenomic (next generation sequencing, NGS) approaches for diagnosis of infection. We undertook a pilot study to screen samples submitted to a diagnostic microbiology laboratory in a UK teaching hospital using Illumina HiSeq. In the short-term, this small dataset provides insights into the virome of human respiratory and cerebrospinal fluid (CSF) samples. In the longer term, assimilating metagenomic data sets of this nature can inform optimization of laboratory and bioinformatic methods, and develop foundations for the interpretation of results in a clinical context. The project underpins a larger ongoing effort to develop NGS pipelines for diagnostic use. DATA DESCRIPTION: Our data comprise a complete metagenomic dataset from 20 independent samples (10 CSF and 10 respiratory) submitted to the clinical microbiology laboratory for a large UK teaching hospital (Oxford University Hospitals NHS Foundation Trust). Sequences have been uploaded to the European Nucleotide Archive and are also presented as Krona plots through which the data can be interactively visualized. In the longer term, further optimization is required to better define sensitivity and specificity of this approach to clinical samples.

Original publication

DOI

10.1186/s13104-018-3234-8

Type

Journal article

Journal

BMC Res Notes

Publication Date

09/02/2018

Volume

11

Keywords

CSF, Diagnosis, Illumina, Infection, Metagenomics, Microbiome, Next generation sequencing, Respiratory, Virome, Cerebrospinal Fluid, High-Throughput Nucleotide Sequencing, Humans, Metagenomics, Pilot Projects, Sequence Analysis, DNA, Sputum