Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The increasing demand for production and characterization of diverse groups of recombinant proteins necessitates the analysis of several constructs and fusion tags in a variety of expression systems. The challenge is to screen multiple clones quickly for the desired properties. When using a eukaryotic system, such as baculovirus-mediated expression in insect cells, the total time required and the volume of culture needed to obtain reasonable results are limiting factors. This chapter focuses on addressing these issues by describing rapid small-scale expression as a mode of screening. The method allows the rapid identification of the best clone before scaling-up and the production of heterologous protein.

Original publication

DOI

10.1007/978-1-59745-188-8_19

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2008

Volume

439

Pages

269 - 289

Addresses

Structural Genomics Consortium, Botnar Research Centre, University of Oxford, Oxford, UK.

Keywords

Cell Line, Animals, Spodoptera, Baculoviridae, Recombinant Proteins, DNA, Viral, DNA Primers, Polymerase Chain Reaction, Base Sequence, Genetic Vectors