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A yeast two-hybrid screen was performed to find new factors involved in pre-mRNA splicing. Using SC35 as a bait, we isolated a human cDNA bearing high homology to the Drosophila transformer-2 (TRA-2) protein. This cDNA was named htra2-beta1. htra2-beta1 is a nuclear protein that colocalizes with SC35 in a speckled pattern. It interacts with several SR proteins tested in yeast. A second form named htra2-beta2 is generated by alternative splicing. This isoform gives rise to a truncated protein without an SR domain. Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these two isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in regulation of htra2-beta activity.

Original publication

DOI

10.1089/dna.1997.16.679

Type

Journal article

Journal

DNA and cell biology

Publication Date

06/1997

Volume

16

Pages

679 - 690

Addresses

Max-Planck Institute for Psychiatry, Planegg, Germany.

Keywords

Cell Line, Animals, Humans, Rats, Drosophila, Saccharomyces cerevisiae, Ribonucleoproteins, Drosophila Proteins, Cloning, Molecular, Alternative Splicing, Amino Acid Sequence, Base Sequence, Sequence Homology, Nucleic Acid, Molecular Sequence Data