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In the present study we evaluated the role of B cells in acquired immunity to Salmonella infection by using gene-targeted B-cell-deficient innately susceptible mice on a C57BL/6 background (Igh-6(-/-)). Igh-6(-/-) mice immunized with a live, attenuated aroA Salmonella enterica serovar Typhimurium vaccine strain showed impaired long-term acquired resistance against the virulent serovar Typhimurium strain C5. Igh-6(-/-) mice were able to control a primary infection and to clear the inoculum from the reticuloendothelial system. However, Igh-6(-/-) mice, unlike Igh-6(+/+) C57BL/6 controls, did not survive an oral challenge with strain C5 at 4 months after vaccination. Transfer of immune serum did not restore resistance in Igh-6(-/-) mice. Total splenocytes and purified CD4(+) T cells obtained from Igh-6(-/-) mice 4 months after vaccination showed reduced ability to release Th1-type cytokines (interleukin 2 and gamma interferon) upon in vitro restimulation with serovar Typhimurium soluble cell extracts compared to cells obtained from Igh-6(+/+) C57BL/6 control mice. Therefore, the impaired resistance to oral challenge with virulent serovar Typhimurium observed in B-cell-deficient mice, which cannot be restored by passive transfer of Salmonella-immune serum, may be in part due to a reduced serovar Typhimurium-specific T-cell response following primary immunization.

Type

Journal article

Journal

Infect Immun

Publication Date

01/2000

Volume

68

Pages

46 - 53

Keywords

Administration, Oral, Animals, Antigens, Bacterial, B-Lymphocytes, Bacterial Vaccines, Female, Genes, Immunoglobulin, Immunization, Immunization, Passive, Immunoglobulin Heavy Chains, Interferon-gamma, Interleukin-2, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Salmonella Infections, Animal, Salmonella typhimurium, Th1 Cells, Vaccines, Attenuated, Virulence