This project will use a model of type 1 diabetes to investigate the impact of microbiome alterations on the immune system and on the cumulative incidence of diabetes. This work will use cutting edge techniques including single cell RNA-sequencing to dissect the relationship between host genome, microbiome and autoimmunity. The project will have a particular focus on understanding the function(s) of the novel type 1 diabetes gene DEXI, building upon the work in this manuscript: https://www.biorxiv.org/content/early/2018/08/16/393421
The training opportunities incude working with an in vivo model; single cell RNA-seq and bulk RNA-seq analysis; microbiome analysis; standard laboratory techniques including DNA and RNA extraction, PCR, western blotting, ELISA, tissue culture, qPCR; flow cytometry; working with large data sets e.g. metabolomics.
Genetics & Genomics and Endocrinology & Metabolic Medicine
Project reference number: 994
|Professor Chris A O'Callaghan||Centre for Cellular and Molecular Physiology||Oxford University, Henry Wellcome Building for Molecular Physiology||GBRfirstname.lastname@example.org|
|Professor Lucy Davison||Nuffield Dept Medicine||University of Oxford||GBRemail@example.com|
There are no publications listed for this DPhil project.