Genes and environment in type 1 diabetes

Project Overview

This project will use a model of type 1 diabetes to investigate the impact of microbiome alterations on the immune system and on the cumulative incidence of diabetes. This work will use cutting edge techniques including single cell RNA-sequencing to dissect the relationship between host genome, microbiome and autoimmunity. The project will have a particular focus on understanding the function(s) of the novel type 1 diabetes gene DEXI, building upon the work in this manuscript: https://www.biorxiv.org/content/early/2018/08/16/393421

Training Opportunities

The training opportunities incude working with an in vivo model; single cell RNA-seq and bulk RNA-seq analysis; microbiome analysis; standard laboratory techniques including DNA and RNA extraction, PCR, western blotting, ELISA, tissue culture, qPCR; flow cytometry; working with large data sets e.g. metabolomics.

Theme

Genetics & Genomics and Endocrinology & Metabolic Medicine

Admissions

Project reference number: 994

Funding and admissions information

Supervisors

Name Department Institution Country Email
Professor Chris A O'Callaghan Centre for Cellular and Molecular Physiology Oxford University, Henry Wellcome Building for Molecular Physiology GBR chris.ocallaghan@ndm.ox.ac.uk
Professor Lucy Davison Nuffield Dept Medicine University of Oxford GBR ldavison@well.ox.ac.uk

There are no publications listed for this DPhil project.