The active form of vitamin B6, namely pyridoxal 5’-phosphate (PLP), is a versatile enzyme catalyst with a double-edged sword. On one hand PLP is an essential cofactor for >4% of all enzymes. On the other, PLP imbalance in the cell is the cause of several inherited epilepsy disorders, and is associated with increased risk in cancer, cardiovascular and other common diseases. The cellular level of PLP therefore needs to be tightly controlled. How this homeostasis is achieved is an important, yet poorly investigated question.
We hypothesize that the biosynthesis, processing and delivery of PLP is mediated via an intricate interplay of diverse cellular proteins. This DPhil project will combine biochemical, structural and proteomic approaches in order to construct an integrated picture of B6 metabolism and homeostasis in the human cell.
The experimental objectives are to:
gain a solid grounding in cutting edge structural biology techniques for the study of enzymes and protein-protein interactions
translate basic science findings into more in vivo context using relevant cellular assays
contribute to an emerging field in understanding genetic disease mutations to shed lights on drug discovery
engage directly with our cell biology and clinical collaborators
work in a multi-disciplinary team, manage experiments, and present data in papers/conferences
Protein Science & Structural Biology and Endocrinology & Metabolic Medicine
Project reference number: 1002
|Associate Professor Wyatt W Yue||Structural Genomics Consortium||Oxford University, Old Road Campus Research Building||GBRemail@example.com|
|Dr Kilian Huber||Structural Genomics Consortium||Oxford University, NDM Research Building||GBRfirstname.lastname@example.org|
There are no publications listed for this DPhil project.