Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Five hapten-modified autogenous mouse serum albumins (MSA), TNP11MSA, FITC8MSA, DNP64MSA, DNP5MSA, and PC2MSA, were tested for their ability to induce hapten-specific antibody responses in H-2 congenic mice of the H2d, H-2b, H-2a, and H-2f haplotypes. Four of the five modified MSAs, TNP11MSA, FITC8MSA, DNP64MSA, and PC2MSA, stimulated responses found to be under H-2-linked Ir gene control, whereas DNP5MSA failed to induce responses in any strain tested. H-2d, H-2D, and H-2a mice responded to FITC8MSA; H-2d and H-2b responded to TNP11MSA; and only H-2d mice were high responders to DNP64MSA and PC2MSA. H-2f mice failed to respond to any of the stimulating antigens. Thus, a hierarchy of H-2 haplotypes was observed in the responsiveness to modified MSA with H-2d greater than H-2b greater than H-2a greater than H-2f. Primed lymph node cells from PC2MSA immunized H-2d mice were challenged with antigen in vitro to assess the nature of the determinant recognized by T cells in the response to modified MSA. Lymph node cells proliferated only when challenged with PC2MSA. Unmodified MSA or TNP11MSA did not stimulate a proliferative response, suggesting that the immune T cells recognize a neodeterminant present on the modified PC2MSA.

Original publication

DOI

10.1016/0145-305x(84)90049-1

Type

Journal article

Journal

Developmental and comparative immunology

Publication Date

01/1984

Volume

8

Pages

425 - 434

Keywords

Lymph Nodes, T-Lymphocytes, Animals, Mice, Inbred Strains, Mice, Serum Albumin, H-2 Antigens, Epitopes, Haptens, Lymphocyte Activation, Genes, MHC Class II