DNA assembly methods are essential for multiple applications including synthetic biology. We recently developed MetClo, a method that uses a single type IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.
Methods in molecular biology (Clifton, N.J.)
143 - 159
Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.