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Understanding the interactions involved during the immunological synapse between peptide, HLA-E molecules, and TCR is crucial to effectively target protective HLA-E-restricted T-cell responses in humans. Here we describe three techniques based on the generation of MHC-E/peptide complexes (MHC-E generically includes HLA-E-like molecules in human and nonhuman species, while HLA-E specifically refers to human molecules), which allow to investigate MHC-E/peptide binding at the molecular level through binding assays and by using peptide loaded HLA-E tetramers, to detect, isolate, and study peptide-specific HLA-E-restricted human T-cells.

Original publication

DOI

10.1007/978-1-0716-2712-9_2

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2022

Volume

2574

Pages

15 - 30

Addresses

Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.

Keywords

T-Lymphocytes, Humans, Peptides, Histocompatibility Antigens Class I, Epitopes