Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells.

Original publication




Journal article


Methods Mol Biol

Publication Date





187 - 207


Animals, Antigen Presentation, COS Cells, Cell Line, Cercopithecus aethiops, Escherichia coli, Gene Library, HEK293 Cells, Histocompatibility Antigens Class I, Humans, Peptide Fragments, Phosphorylation, T-Lymphocytes, Cytotoxic, Transfection, Transformation, Genetic, Tumor Necrosis Factor-alpha