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We have evaluated the influence of age and immunization routes for induction of HIV-1- and M. tuberculosis-specific immune responses after neonatal (7 days old) and adult (7 weeks old) BALB/c mice immunization with BCG.HIVA(222) prime and MVA.HIVA boost. The specific HIV-1 cellular immune responses were analyzed in spleen cells. The body weight of the newborn mice was weekly recorded. The frequencies of HIV-specific CD8(+) T cells producing IFN-γ were higher in adult mice vaccinated intradermally and lower in adult and newborn mice vaccinated subcutaneously. In all cases the IFN-γ production was significantly higher when mice were primed with BCG.HIVA(222) compared with BCGwt. When the HIV-specific CTL activity was assessed, the frequencies of specific killing were higher in newborn mice than in adults. The prime-boost vaccination regimen which includes BCG.HIVA(222) and MVA.HIVA was safe when inoculated to newborn mice. The administration of BCG.HIVA(222) to newborn mice is safe and immunogenic and increased the HIV-specific responses induced by MVA.HIVA vaccine. It might be a good model for infant HIV and Tuberculosis bivalent vaccine.

Original publication

DOI

10.1155/2011/516219

Type

Journal article

Journal

Clin Dev Immunol

Publication Date

2011

Volume

2011

Keywords

AIDS Vaccines, Adaptive Immunity, Age Factors, Animals, Animals, Newborn, BCG Vaccine, CD8-Positive T-Lymphocytes, Drug Administration Routes, Epitopes, Female, HIV Infections, HIV-1, Mice, Mice, Inbred BALB C, Tuberculin, Vaccination