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We tested a phenyl mannitol broth containing ceftizoxime and aztreonam (PHMB(+)) for detection of methicillin-resistant Staphylococcus aureus (MRSA) with reference MRSA strains and, subsequently, with clinical samples (n = 1,098). All reference MRSA strains induced color change in PHMB(+) after 24 to 72 h of incubation. In a clinical setting, 40 MRSA strains were detected with PHMB(+), compared with only 23 detected with a routine method. Thus, this selective broth significantly (P < 0.001) improved the rate of MRSA detection.

Original publication

DOI

10.1128/JCM.39.7.2660-2662.2001

Type

Journal article

Journal

J Clin Microbiol

Publication Date

07/2001

Volume

39

Pages

2660 - 2662

Keywords

Aztreonam, Bacteriological Techniques, Ceftizoxime, Cephalosporins, Culture Media, Humans, Mannitol, Methicillin Resistance, Monobactams, Phenolsulfonphthalein, Staphylococcal Infections, Staphylococcus aureus