Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit.
Choudhurry RSR., Melles DC., Eadie K., Vos M., Wertheim HFL., Verbrugh HA., van Belkum A., van Leeuwen WB.
The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.