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The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.

Original publication

DOI

10.1016/j.mimet.2005.08.009

Type

Journal article

Journal

J Microbiol Methods

Publication Date

05/2006

Volume

65

Pages

354 - 356

Keywords

Carrier State, Humans, Nasal Cavity, Polymerase Chain Reaction, Reagent Kits, Diagnostic, Sensitivity and Specificity, Staphylococcal Infections, Staphylococcus aureus