Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate

Yuan F., Bi Y., Siejka-Zielinska P., Zhou Y-L., Zhang X-X., Song C-X.

WO-Seq: a bisulfite-free and base-resolution sequencing method based on peroxotungstate oxidation is presented for the identification of hm5C sites in the transcriptome. Combining the peroxotungstate oxidation with TET enzyme oxidation, m5C can also be detected in a procedure termed TET-Assisted WO-Seq (TAWO-Seq).

DOI

10.1039/c9cc00274j

Type

Journal article

Journal

Chemical Communications

Publisher

Royal Society of Chemistry (RSC)

Publication Date

2019

Volume

55

Pages

2328 - 2331

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