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We have developed a method for intact mass analysis of detergent-solubilized and purified integral membrane proteins using liquid chromatography-mass spectrometry (LC-MS) with methanol as the organic mobile phase. Membrane proteins and detergents are separated chromatographically during the isocratic stage of the gradient profile from a 150-mm C3 reversed-phase column. The mass accuracy is comparable to standard methods employed for soluble proteins; the sensitivity is 10-fold lower, requiring 0.2-5 μg of protein. The method is also compatible with our standard LC-MS method used for intact mass analysis of soluble proteins and may therefore be applied on a multiuser instrument or in a high-throughput environment.

Original publication

DOI

10.1016/j.ab.2010.11.008

Type

Journal article

Journal

Anal Biochem

Publication Date

15/03/2011

Volume

410

Pages

272 - 280

Keywords

Chromatography, Chromatography, High Pressure Liquid, Chromatography, Liquid, Detergents, Mass Spectrometry, Membrane Proteins, Methanol, Spectrometry, Mass, Electrospray Ionization