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We have developed a method for the automatic quantification of chromosome dynamics in live fluorescent microscopy image sequences of mitosis in Drosophila early embryos. Our approach utilises the Fast Level Sets method to perform image segmentation and tracking and incorporates the use of intensity information and prior knowledge of the shape of the chromosomes in the images, the method allows us to easily extract biologically relevant parameters. Tracking and measurements from real fluorescent imaging datasets are presented, highlighting the robustness of our techniques. © 2007 IEEE.

Original publication

DOI

10.1109/ISBI.2007.356839

Type

Conference paper

Publication Date

27/11/2007

Pages

264 - 267