This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the BacMam system. This eukaryotic expression system was selected and a screening process established in 2016 to enable production of highly challenging human integral membrane proteins (IMPs), which are a significant component of our target list. Here, we discuss our recently developed platform for identifying expression and monodispersity of IMPs from 3 mL of HEK293 cells.
Journal article
2021-01-01T00:00:00+00:00
2199
95 - 115
20
Astex Pharmaceuticals, Cambridge, UK.
Humans, Membrane Proteins, Recombinant Proteins, Gene Expression, Genetic Vectors, HEK293 Cells