Use of Methylmalonyl‐CoA Epimerase in Enhancing Crotonase Stereoselectivity
Hamed RB., Gomez‐Castellanos JR., Sean Froese D., Krysztofinska E., Yue WW., Schofield CJ.
AbstractThe use of methylmalonyl‐CoA epimerase (MCEE) to improve stereoselectivity in crotonase‐mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl‐CoA carboxylase reductase and carboxymethylproline synthase in a three‐enzyme one‐pot sequential synthesis of functionalised C‐5 carboxyalkylprolines starting from crotonyl‐CoA and carbon dioxide.