PELDOR Spectroscopy Distance Fingerprinting of the Octameric Outer‐Membrane Protein Wza from Escherichia coli.
Hagelueken G., Ingledew WJ., Huang H., Petrovic‐Stojanovska B., Whitfield C., ElMkami H., Schiemann O., Naismith JH.
AbstractDistance fingerprinting: Pulsed electron–electron double resonance spectroscopy (PELDOR) is applied to the octameric membrane protein complex Wza of E. coli. The data yielded a detailed distance fingerprint of its periplasmic region that compares favorably to the crystal structure. These results provide the foundation to study conformation changes from interaction with partner proteins.magnified image