Simplification of two‐dimensional gel patterns of HLA class II antigens
Rust NA., McMichael AJ.
Two‐dimensional gel electrophoresis of material immunoprecipitated from radiolabeled cells by anti‐HLA class II monoclonal antibodies has been a useful technique but the patterns observed have varied considerably between laboratories. In this study we investigated the parameters that vary between different laboratories. We found that heterogeneity due to post synthetic modification of β and α chains of DR and DQ can be avoided by labeling cells for 30 min with 35S‐methionine. A mixture of ampholytes pH range 3.5–10: 5–7 of 1:4 run for 3000 V/h gave the best resolution of β and α chains. With this technique we could clearly reveal differences in the DR β–2 chains of those DR2 haplotypes, Dw2, Dw12 and LD'tb24′. The DR β1 position varied between different DR types.