Method for Measuring Phenotypic Colistin Resistance in Escherichia coli Populations from Chicken Flocks
Nhung NT., Yen NTP., Thien NVK., Cuong NV., Kiet BT., Campbell J., Thwaites G., Baker S., Geskus RB., Carrique-Mas J.
Colistin (polymyxin E) is an antimicrobial with poor solubility in agar-based media, and therefore, broth microdilution is the only available method for determining phenotypic resistance. However, estimating colistin resistance in mixed Escherichia coli populations is laborious, since it requires individual colony isolation, identification, and susceptibility testing.