Imaging real-time HIV-1 virion fusion with FRET-based biosensors
Jones DM., Padilla-Parra S.
AbstractWe have produced a novel, simple and rapid method utilising genetically encodable FRET-based biosensors to permit the detection of HIV-1 virion fusion in living cells. These biosensors show high sensitivity both spatially and temporally and allow the real-time recovery of HIV-1 fusion kinetics in both single cells and cell populations simultaneously.