Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

This paper describes a general procedure for the two-step purification of recombinant proteins as antibody-antigen complexes in which there is no uncomplexed antibody or antigen. In this way, immune complexes containing the p17, p27, vpr and vpx proteins of simian immunodeficiency virus (SIV) have been purified. Antibody-antigen complexes are more immunogenic than antigen when administered either alone or with alum. The significance of the work is that this general method could be modified for the manufacture of immune complexes for incorporation into multivalent vaccines.

Type

Journal article

Journal

Vaccine

Publication Date

03/1994

Volume

12

Pages

351 - 358

Keywords

Animals, Antigen-Antibody Complex, Antigens, Viral, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Mice, Recombinant Proteins, Simian Immunodeficiency Virus, Viral Proteins