Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We have previously shown that immunization with solid matrix-antigen-antibody (SMAA) complexes induces both vigorous humoral and cell-mediated immune responses and have suggested that this method of vaccination may be developed for use in humans, and potentially as a vaccine against AIDS. Here we demonstrate that a small oligopeptide can act as a tag for the construction of SMAA complexes using a tag-specific monoclonal antibody and tag-linked antigens. We show that a 14-amino acid oligopeptide, present in the phospho (P) and V proteins of simian virus 5 (SV5), retains its antigenicity when attached to the C terminus of three 'foreign' proteins [p27 and gp110 of simian immunodeficiency virus (SIV) and glutathione S-transferase] such that these proteins can be incorporated into SMAA complexes using a monoclonal antibody (MAb) that was originally raised against the native SV5 P and V proteins. Mice were immunized with SMAA complexes containing recombinant p27-TAG and MAbs have been isolated that recognized native SIV p27. The significance of these results in terms of the development of SMAA complexes as human vaccines is discussed.

Original publication

DOI

10.1099/0022-1317-73-3-653

Type

Journal article

Journal

J Gen Virol

Publication Date

03/1992

Volume

73 ( Pt 3)

Pages

653 - 660

Keywords

Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigen-Antibody Complex, Antigens, Viral, Base Sequence, Epitopes, Gene Products, gag, Genetic Vectors, HIV Antigens, Mice, Molecular Sequence Data, Oligopeptides, Paramyxoviridae, Recombinant Proteins, Simian Immunodeficiency Virus, Vaccines, Synthetic, Viral Envelope Proteins