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Protein engineering has an array of uses: whether you are studying a disease mutation, removing undesirable sequences, adding stabilizing mutations for structural purposes, or simply dissecting protein function. Protein engineering is almost exclusively performed using site-directed mutagenesis (SDM) as this provides targeted modification of specific amino acids, as well as the option of rewriting the native sequence to include or exclude certain regions. Despite its widespread use, SDM has often proved to be a bottleneck, requiring precision manipulation on a sample-by-sample basis to make it work. When dealing with large volumes of samples it is not possible to use such a low-throughput approach. Here we describe a high-throughput (HTP) method for SDM, optimized and used by the Structural Genomics Consortium (SGC) to complement structural studies.

Original publication

DOI

10.1007/978-1-4939-9624-7_13

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2019

Volume

2025

Pages

281 - 296

Keywords

High-throughput (HTP), Ligation-independent cloning (LIC), Protein engineering, Site-directed mutagenesis (SDM)