Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes
Lanyon-Hogg T., Ritzefeld M., Sefer L., Bickel JK., Rudolf AF., Panyain N., Bineva-Todd G., Ocasio CA., O'Reilly N., Siebold C., Magee AI., Tate EW.
A highly accurate and versatile fluorescence polarisation assay for any enzyme adding or removing lipid posttranslational modifications, with the potential to accelerate drug discovery against these targets.