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LARP1 is an oncogenic RNA-binding protein required for ribosome biogenesis and cancer cell survival. From published in vitro studies, there is disparity over which of two different LARP1 protein isoforms (termed the long LI-LARP1 and short SI-LARP1) is the canonical. Here, after conducting a series of biochemical and cellular assays, we conclude that LI-LARP1 (NM_033551.3 > NP_056130.2) is the dominantly expressed form. We observe that SI-LARP1 (NM_015315.5> NP_056130.2) is epigenetically repressed and that this repression is evolutionarily conserved in all but a small subclade of mammalian species. As with other LARP family members, there are multiple potential LARP1 mRNA isoforms that appear to be censored within the nucleus. The capacity of the cell to modulate splicing and expression of these apparently 'redundant' mRNAs hints at contextually specific mechanisms of LARP1 expression.

Original publication

DOI

10.1080/15476286.2020.1744320

Type

Journal article

Journal

RNA biology

Publication Date

02/2021

Volume

18

Pages

237 - 247

Addresses

Department of Oncology, University of Oxford, Oxford, UK.

Keywords

Cell Line, Tumor, Humans, RNA-Binding Proteins, Ribonucleoproteins, Protein Isoforms, RNA, Autoantigens, Organ Specificity, DNA Methylation, Gene Expression Regulation, Neoplastic, Gene Silencing, Alternative Splicing, Amino Acid Sequence, Protein Binding, Multigene Family, Promoter Regions, Genetic