Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

MxB/Mx2 is an interferon-induced dynamin-like GTPase, which restricts a number of life-threatening viruses. Because of its N-terminal region, predicted to be intrinsically disordered, and its propensity to self-oligomerize, purification of the full-length protein has not been successful in conventional E. coli expression systems. In this chapter, we describe an expression and purification procedure to obtain pure full-length wild-type MxB from suspension-adapted mammalian cells. We further describe how to characterize its GTPase activity and oligomerization function.

Original publication




Journal article


Methods in molecular biology (Clifton, N.J.)

Publication Date





55 - 65


Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.


Animals, Humans, GTP Phosphohydrolases, Recombinant Fusion Proteins, Chromatography, Affinity, Chromatography, Gel, Transfection, Gene Expression, Enzyme Activation, Plasmids, HEK293 Cells, Myxovirus Resistance Proteins