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Previous studies have documented the ability of 8-bromoguanosine (8-BrGuo) and 8-mercaptoguanosine (8-MGuo) to induce polyclonal proliferation and differentiation of B cells from a variety of mouse strains. In the present study, we have defined the cellular target of this mitogenic activity. Using B cells fractionated according to size, we have found that large B cells are responsive to 8-BrGuo- and 8-MGuo-induced proliferation and differentiation whereas small, resting B cells are relatively unresponsive to these compounds. Addition of splenic adherent cells to the small B-cell fraction partially restored the proliferative but not the differentiative responses to 8-BrGuo and 8-MGuo. Although small B cells alone did not proliferate or differentiate in response to 8-BrGuo and 8-MGuo, cell surface expression of Ia antigens increased following incubation with these compounds. Thus, the biological activity of 8-BrGuo and 8-MGuo appears to be dictated by the cell type upon which it is acting. Small B cells are activated as evidenced by increased levels of surface Ia whereas large B cells are not only activated but are also induced to proliferate and differentiate.

Original publication

DOI

10.1016/0008-8749(87)90175-4

Type

Journal article

Journal

Cellular immunology

Publication Date

05/1987

Volume

106

Pages

318 - 329

Keywords

Spleen, B-Lymphocytes, Macrophages, Animals, Mice, Muramidase, Receptors, Antigen, B-Cell, Guanosine, Thionucleosides, Antibodies, Anti-Idiotypic, Antigens, Surface, Histocompatibility Antigens Class II, Lymphocyte Activation, Cell Division, Cell Differentiation, Antibody Formation