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DNA assembly methods are essential for multiple applications including synthetic biology. We recently developed MetClo, a method that uses a single type IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.

Original publication

DOI

10.1007/978-1-0716-0908-8_9

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2020

Volume

2205

Pages

143 - 159

Addresses

Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford, Oxford, UK.