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The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes of near-native, fully hydrated frozen cells and tissues at voxel sizes of 10 nm and below. We explored this capability for pathologic characterization of vitrified human patient cells by developing and optimizing a serial cryoFIB/SEM volume imaging workflow. We demonstrate profound disruption of subcellular architecture in primary fibroblasts from a Leigh syndrome patient harboring a disease-causing mutation in USMG5 protein responsible for impaired mitochondrial energy production.

Original publication

DOI

10.1016/j.str.2020.10.003

Type

Journal article

Journal

Structure (London, England : 1993)

Publication Date

01/2021

Volume

29

Pages

82 - 87.e3

Addresses

Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK.

Keywords

Cells, Cultured, Mitochondria, Fibroblasts, Humans, Leigh Disease, Mitochondrial Proton-Translocating ATPases, Cryoelectron Microscopy, Mutation, Primary Cell Culture