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Structural genomics groups have identified the need to generate multiple truncated versions of each target to improve their success in producing a well-expressed, soluble, and stable protein and one that crystallizes and diffracts to a sufficient resolution for structural determination. At the Structural Genomics Consortium, we opted for the ligation-independent cloning (LIC) method which provides the throughput we desire to produce and screen many proteins in a parallel process. Here, we describe our LIC protocol for generating constructs in 96-well format and provide a choice of vectors suitable for expressing proteins in both E. coli and the baculovirus expression vector system (BEVS).

Original publication




Journal article


Methods in molecular biology (Clifton, N.J.)

Publication Date





23 - 43


Diamond Light Source Ltd., Didcot, Oxfordshire, UK.


Humans, Escherichia coli, Baculoviridae, Recombinant Proteins, Cloning, Molecular, Gene Expression, Genetic Vectors