Mahajan P., Ellis K., Mukhopadhyay S., Fernandez-Cid A., Chi G., Man H., Dürr KL., Burgess-Brown NA.

This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the BacMam system. This eukaryotic expression system was selected and a screening process established in 2016 to enable production of highly challenging human integral membrane proteins (IMPs), which are a significant component of our target list. Here, we discuss our recently developed platform for identifying expression and monodispersity of IMPs from 3 mL of HEK293 cells.

Expression Screening of Human Integral Membrane Proteins Using BacMam.

Mahajan P., Ellis K., Mukhopadhyay S., Fernandez-Cid A., Chi G., Man H., Dürr KL., Burgess-Brown NA.

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