Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test.
Amornchai P., Chierakul W., Wuthiekanun V., Mahakhunkijcharoen Y., Phetsouvanh R., Currie BJ., Newton PN., van Vinh Chau N., Wongratanacheewin S., Day NPJ., Peacock SJ.
In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli.