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Another method has been developed to attach synthetic peptides to solid supports for use in enzyme immunoassays. The method is based on passively adsorbing a synthetic peptide to a solid-phase support, then further attaching more of the same peptide by means of cross-linking to the previously adsorbed peptide. This method results in highly enhanced peptide immunoreactivity compared with that obtained with standard methodologies.

Type

Journal article

Journal

BioTechniques

Publication Date

11/1992

Volume

13

Pages

738 - 743

Addresses

Baxter Diagnostics, Inc., Pandex Division, Mundelein, IL 60060-3021.

Keywords

Humans, Hepacivirus, Polystyrenes, Peptides, Antigens, Viral, Cross-Linking Reagents, Immunoenzyme Techniques, Immunologic Techniques, Microspheres, Amino Acid Sequence, Surface Properties, Magnetics, Molecular Sequence Data