Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Summary This study demonstrated that in‐situ hybridization using digoxigenin‐labelled oligonucleotide poIy‐T probes and digoxigenin‐labelled riboprobes for keratin 14 is possible on routinely fixed paraffin‐embedded sections. The resolution and time course of the detection system compared favourably with isotopically labelled probes. Major differences were shown in keratinocyte messenger RNA content with differentiating cells expressing high levels when compared with the basal layers. Previous work on the distribution of keratin 14 messenger RNA was confirmed and we were able to show that using non‐isotopic in‐situ hybridization, sensitive and accurate cellular localization was feasible in skin sections. Copyright © 1991, Wiley Blackwell. All rights reserved

Original publication

DOI

10.1111/j.1365-2133.1991.tb14787.x

Type

Journal article

Journal

British Journal of Dermatology

Publication Date

01/01/1991

Volume

125

Pages

516 - 520