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African swine fever virus is a cytolytic virus that leads to the apoptosis of both cultured cells and primary macrophages. Cell culture supernatants of virus-infected cells are routinely used for virological and immunological studies, despite differences in the biological behavior between such preparations and highly purified virus. In addition, more recent data suggests that exosomes containing viral proteins may be secreted from infected cells. While African swine fever virus can be purified through a number of methods, in our hands Percoll provides the most robust method of separating virus from cellular contaminants.

Original publication

DOI

10.1007/978-1-0716-2333-6_13

Type

Journal article

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2022

Volume

2503

Pages

179 - 186

Addresses

African Swine Fever Vaccinology Group, The Pirbright Institute, Pirbright, Woking, UK.

Keywords

Cells, Cultured, Cell Line, Animals, Swine, DNA Viruses, African Swine Fever Virus, African Swine Fever, Viral Proteins