Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The Tp53 gene is a well-known tumour suppressor, mutation of which (e.g. prevention of Ser312 phosphorylation) induces deletion or expression of an inactive p53 protein to increase the susceptibility of tumour occurance. However, the role of Tp53 gene in maintaining metabolic homeostasis for regulating physio-pathological activities is still not well-understood. This study aimed to use the lipidomics study as a systematic approach to understand the relationship between the phenotypic effects of Tp53 mutation on lipid-related endogenous metabolites. Plasma and liver samples from mice carrying a Tp53 Ser312 to Ala mutation and wild type mice were collected, lipids were extracted by liquid-liquid extraction method and analyzed by the RPLC-LTQ-FTMS for the lipidomics study. Our results indicated that defect in Ser312 phosphorylation of Tp53 leads the lipid disturbance (e.g. triacylglycerols) for fatty accumulation and fatty liver susceptibility, which is with preference of females. Histological observation by staining with haematoxylin and eosin further validated our lipidomics findings. To our conclusion, fatty liver occurrence may have different phenotypes, one of which is strongly linked with the Tp53 mutation and is susceptible in females. Lipidomics as a technique to detect a great number of endogenous compounds provides precise metabolic information that may further help improve personalized diagnosis of Chronic hepatic diseases.

Original publication




Journal article


Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Publication Date





Northeast Asian Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, No. 1035, Boshuo Rd, Jingyue Economic Development District, 130117 Changchun, PR China; Leiden University-European Center for Chinese Medicine and Natural Compounds, Institute of Biology Leiden (IBL), Leiden University, Sylviusweg 72, 2333BE Leiden, the Netherlands.