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Crystals of bacteriophage PRD1, a virus containing an internal lipid bilayer, have been grown in thin-walled quartz capillary tubes by vapour diffusion as a means of eliminating mechanical handling of the crystals during data collection. It has been found that the addition of polyethylene glycol 20 000 (PEG 20K) to the mother liquor that bathes the crystals allows far higher resolution diffraction intensities to be observed. Growing and treating the crystals in this way has produced a small number of crystals which are particularly amenable to X-ray diffraction analysis.

Type

Journal article

Journal

Acta Crystallogr D Biol Crystallogr

Publication Date

03/2003

Volume

59

Pages

538 - 540

Keywords

Bacteriophage PRD1, Crystallization, Membranes, Quartz, Salmonella enterica, X-Ray Diffraction