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Dengue fever, a mosquito-borne viral disease of significant public health concern in tropical and subtropical regions, is caused by any of the four serotypes of the dengue virus (DENV1-4). Cutting-edge technologies like next-generation sequencing (NGS) are revolutionizing virology, enabling in-depth exploration of DENV's genetic diversity. Here, we present an optimized workflow for full-genome sequencing of DENV 1-4 utilizing tiled amplicon multiplex PCR and Illumina sequencing. Our assay, sequenced on the Illumina MiSeq platform, demonstrates its ability to recover the full-length dengue genome across various viral abundances in clinical specimens with high-quality base coverage. This high quality underscores its suitability for precise examination of intra-host diversity, enriching our understanding of viral evolution and holding potential for improved diagnostic and intervention strategies in regions facing dengue outbreaks.

Original publication

DOI

10.1016/j.jviromet.2024.114968

Type

Journal article

Journal

Journal of virological methods

Publication Date

07/2024

Volume

328

Addresses

Oxford University Clinical Research Unit, Wellcome Trust Africa Asia Programme, District 5, Ho Chi Minh City, Viet Nam.

Keywords

Humans, Dengue Virus, Dengue, RNA, Viral, Genome, Viral, High-Throughput Nucleotide Sequencing, Multiplex Polymerase Chain Reaction, Serogroup, Whole Genome Sequencing