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NK cells lyse hematopoietic cells that lack expression of MHC class I molecules on the cell surface. Transfection of certain MHC class I negative cell lines with MHC class I genes renders these cells resistant to NK cell-mediated cytotoxicity. Recently, we described an NK cell receptor, NKB1, that inhibits NK cells from killing target cells expressing Bw4-reactive HLA-B molecules (-B*2705, -B*5101, -B*5801). In this study, we have demonstrated that another structurally distinct NK cell membrane glycoprotein, HP-3E4, is involved in the recognition of certain polymorphic HLA-C molecules (-Cw*0401 and -Cw*1503). NK cell clones co-expressing both the NKB1 and HP-3E4 receptors fail to lyse targets expressing HLA-Cw*0401 and -B*5801, but are able to kill the transfectants in the presence of mAbs against both receptors. These studies demonstrate that a single NK cell clone may possess multiple structurally distinct receptors for different polymorphic HLA class I molecules that function independently.

Type

Journal article

Journal

Journal of immunology (Baltimore, Md. : 1950)

Publication Date

04/1995

Volume

154

Pages

3320 - 3327

Addresses

Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304.

Keywords

Killer Cells, Natural, Humans, Glycoproteins, Receptors, Immunologic, Antibodies, Monoclonal, HLA-B Antigens, HLA-C Antigens, Cytotoxicity Tests, Immunologic, Flow Cytometry, Transfection, Adult, Receptors, KIR, Receptors, KIR3DL1