Figure 3 from Dynamic Tracking of Tumor Microenvironment Modulation Using Kaede Photoconvertible Transgenic Mice Unveils New Biological Properties of Viral Immunotherapy

<p>Expansion of immune cells within tumors is, in part, due to CAN-2409 + prodrug–mediated stimulation of proliferation of T-cell subsets in the TME (total, neoantigen-specific, and stem-like CD8⁺ T cells). <b>A,</b> The effect of CAN-2409 + prodrug treatment on proliferative status of T-cell subsets was assessed using Ki67 as a marker. Representative flow cytometry plots for total, KG⁺, and KR⁺ populations of total, TAA–specific (MC38 pentamer⁺), and stem-like (PD-1⁺Ly108⁺) CD8⁺ T cells and quantification are shown in <b>B–D</b>. <i>N</i> = 4–6 mice per group. Two-tailed <i>t</i> test, *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001.</p>