Figure 5 from Dynamic Tracking of Tumor Microenvironment Modulation Using Kaede Photoconvertible Transgenic Mice Unveils New Biological Properties of Viral Immunotherapy
Diers AR., Guo Q., Li Z., Richardson E., Idris S., Willis C., Tak PP., Withers DR., Barone F.
<p>CAN-2409 + prodrug treatment reinvigorates tumor-retained, exhausted CD8<sup>+</sup> T cells, and tumor-experienced CD8<sup>+</sup> T cells, which egress tumors, have enhanced functionality. The effect of CAN-2409 + prodrug treatment on terminal exhaustion of CD8<sup>+</sup> T cells was assessed in the tumor using CX3CR1 as a marker coupled with the granzyme B expression. <b>A,</b> Representative flow cytometry plots for total, KG<sup>+</sup>, and KR<sup>+</sup> populations of total and TAA-specific (MC38 pentamer<sup>+</sup>) CD8<sup>+</sup> T cells and quantification are shown in <b>B</b>. <i>N</i> = 4–6 mice per group. Two-tailed <i>t</i> test, *, <i>P</i> < 0.05.</p>