Figure 6 from Dynamic Tracking of Tumor Microenvironment Modulation Using Kaede Photoconvertible Transgenic Mice Unveils New Biological Properties of Viral Immunotherapy
Diers AR., Guo Q., Li Z., Richardson E., Idris S., Willis C., Tak PP., Withers DR., Barone F.
<p>Tumor-recruited, tumor-retained, and tumor-experienced Tregs, which egress tumors, adopt an altered phenotype after CAN-2409 + prodrug treatment. The effect of CAN-2409 + prodrug treatment on proliferative status of Treg cells was assessed using Ki67 as a marker coupled with the PD-1 expression. <b>A,</b> Representative flow cytometry plots for total, KG<sup>+</sup>, and KR<sup>+</sup> Treg cells. Quantification of tumor experiencing Treg (KR<sup>+</sup>) for proliferation status (Ki67), <b>B</b>, and immune suppressive functional markers (PD-1, <b>C</b>, <i>N</i> = 4–6. Two-tailed <i>t</i> test, *, <i>P</i> < 0.05; ***, <i>P</i> < 0.001.</p>