Figure 2 from Dynamic Tracking of Tumor Microenvironment Modulation Using Kaede Photoconvertible Transgenic Mice Unveils New Biological Properties of Viral Immunotherapy
Diers AR., Guo Q., Li Z., Richardson E., Idris S., Willis C., Tak PP., Withers DR., Barone F.
<p>CAN-2409 + prodrug induces expansion of immune cells and neoantigen-specific T cells retained in tumors. <b>A,</b> Summary of MC38 implanted subcutaneous on the flank of C57BL/6 Kaede mice and photoconverted by violet light exposure. <b>B,</b> Study timeline for experiments assessing the effect of CAN-2409 + prodrug on TME modulation in Kaede photoconvertible mice. <b>C,</b> Representative flow cytometry plots showing KR<sup>+</sup> and KG<sup>+</sup> expression in immune subpopulations within tumors. MC38 pentamers were used to identify antigen-specific CD8<sup>+</sup> T cells. <b>D,</b> Quantification of newly entering (KG<sup>+</sup>) cells for each T-cell subset. <i>N</i> = 4–6 mice per group. Two-tailed <i>t</i> test, *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001.</p>