Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

An adaptive immune response is initiated by mature dendritic cells presenting processed antigen to nai;ve T cells. Assuming that the magnitude of the immune response is influenced by the number and type of antigen-presenting dendritic cells and by the duration of antigen presentation, we tested if chemokines that bind to receptors expressed on immature dendritic cells or TRANCE, a survival factor for mature dendritic cells, can serve as adjuvants. None of the immunomodulaters given as genetic adjuvants with a DNA vaccine encoding the full-length rabies virus glycoprotein augmented the transgene product-specific response. However, RANTES, MCP-1, MIP 1-beta, and TRANCE given together with a DNA vaccine expressing a truncated and thus secreted version of the rabies virus glycoprotein enhanced the response suggesting that the tested genetic adjuvants promoted preferentially presentation of reprocessed antigen originating from transduced tissue cells.

Type

Journal article

Journal

Cell Immunol

Publication Date

08/2003

Volume

224

Pages

106 - 113

Keywords

Animals, Antibodies, Viral, Carrier Proteins, Chemokines, Female, Genetic Vectors, Immunoglobulin G, Interferon-gamma, Interleukin-2, Membrane Glycoproteins, Mice, Mice, Inbred C3H, RANK Ligand, Rabies Vaccines, Receptor Activator of Nuclear Factor-kappa B, Vaccines, DNA