Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Three types of vaccine, all expressing the same antigen from Plasmodium berghei, or a CD8+ T cell epitope from that antigen, were compared for their ability to induce CD8+ T cell responses in mice. Higher levels of lysis and numbers of IFN-gamma secreting T cells were primed with Ty virus-like particles and Modified Vaccinia Virus Ankara (MVA) than with DNA vaccines, but none of the vaccines were able to protect immunised mice from infectious challenge even after repeated doses. However, when the immune response was primed with one type of vaccine (Ty-VLPs or DNA) and boosted with another (MVA) complete protection against infection was achieved. Protection correlated with very high levels of IFN-gamma secreting T cells and lysis. This method of vaccination uses delivery systems and routes that can be used in humans and could provide a generally applicable regime for the induction of high levels of CD8+ T cells.

Original publication

DOI

10.1515/BC.1999.041

Type

Journal article

Journal

Biol Chem

Publication Date

03/1999

Volume

380

Pages

299 - 303

Keywords

Animals, Antigens, Protozoan, Female, Malaria, Malaria Vaccines, Mice, Mice, Inbred BALB C, Plasmodium berghei, Protozoan Proteins, Recombinant Fusion Proteins, Retroelements, Vaccines, DNA, Vaccinia virus, Virion